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sheep polyclonal af6788  (R&D Systems)


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    R&D Systems sheep polyclonal af6788
    Sheep Polyclonal Af6788, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+nlrp1+polyclonal+antibody+af6788/us12570735-950-61-59?v=R%26D+Systems
    Average 94 stars, based on 21 article reviews
    sheep polyclonal af6788 - by Bioz Stars, 2026-08
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    FIGURE 1 PM2.5 enhances NLRP1 expression in A549 cells. Expression of inflammasome-related PRRs in A549 cells. A549 cells were exposed to PM2.5 at 2 µg/mL for four days. PM2.5 was dissolved in deionized water to produce 20 µg/mL stock solutions, which were then added to the cell culture at the final concentration. Data are presented as the mean ± standard error. n = 3 independent experiments. *p<0.05; N.D, not detected. Control vehicle-treated control.

    Journal: Frontiers in immunology

    Article Title: Benzo[ a ]pyrene induces NLRP1 expression and promotes prolonged inflammasome signaling.

    doi: 10.3389/fimmu.2023.1154857

    Figure Lengend Snippet: FIGURE 1 PM2.5 enhances NLRP1 expression in A549 cells. Expression of inflammasome-related PRRs in A549 cells. A549 cells were exposed to PM2.5 at 2 µg/mL for four days. PM2.5 was dissolved in deionized water to produce 20 µg/mL stock solutions, which were then added to the cell culture at the final concentration. Data are presented as the mean ± standard error. n = 3 independent experiments. *p<0.05; N.D, not detected. Control vehicle-treated control.

    Article Snippet: Monoclonal and polyclonal antibodies against NLRP1 (R&D Systems, Minneapolis, MN, USA), Frontiers in Immunology 03 NLRP3 (Abclonal Biotechnology, Wuhan, China), AhR (BioLegend, San Diego, CA), ASC (Abclonal Biotechnology), procaspase-1, p20, p53 (R&D Systems), phosphorylated p53 (Ser 37, Cell Signaling, Beverly, MA, USA), actin, and vinculin were primary antibodies.

    Techniques: Expressing, Cell Culture, Concentration Assay, Control

    FIGURE 2 BaP enhances NLRP1 expression in A549 cells. BaP was dissolved in DMSO (final concentration, 0.05%) and added to A549 cells. (A) Expression of inflammasome-related PRRs in A549 cells. The cells were treated with 2 mM BaP for four days. (B) NLRP1 expression followed by BaP (0–2000 nM) for 0–4 days. (C) NLRP1 protein expression levels after four days of BaP treatment (2000 nM). Data are presented as the mean ± standard error. n = 4–9 independent experiments. **p<0.01, ***p<0.001; Control vehicle-treated control.

    Journal: Frontiers in immunology

    Article Title: Benzo[ a ]pyrene induces NLRP1 expression and promotes prolonged inflammasome signaling.

    doi: 10.3389/fimmu.2023.1154857

    Figure Lengend Snippet: FIGURE 2 BaP enhances NLRP1 expression in A549 cells. BaP was dissolved in DMSO (final concentration, 0.05%) and added to A549 cells. (A) Expression of inflammasome-related PRRs in A549 cells. The cells were treated with 2 mM BaP for four days. (B) NLRP1 expression followed by BaP (0–2000 nM) for 0–4 days. (C) NLRP1 protein expression levels after four days of BaP treatment (2000 nM). Data are presented as the mean ± standard error. n = 4–9 independent experiments. **p<0.01, ***p<0.001; Control vehicle-treated control.

    Article Snippet: Monoclonal and polyclonal antibodies against NLRP1 (R&D Systems, Minneapolis, MN, USA), Frontiers in Immunology 03 NLRP3 (Abclonal Biotechnology, Wuhan, China), AhR (BioLegend, San Diego, CA), ASC (Abclonal Biotechnology), procaspase-1, p20, p53 (R&D Systems), phosphorylated p53 (Ser 37, Cell Signaling, Beverly, MA, USA), actin, and vinculin were primary antibodies.

    Techniques: Expressing, Concentration Assay, Control

    FIGURE 3 AhR knockdown by siRNA abrogates the BaP-induced NLRP1 expression. AhR participation in the enhanced NLRP1 expression in BaP-treated A549 cells was determined using AhR KD cells and an AhR antagonist. (A) AhR mRNA and protein expression in response to specific siRNAs. (B) NLRP1 expression after 2 µM BaP exposure for two (mRNA) or four (protein) days in AhR-knockdown cells and control cells. (C) Pro-IL-1b, ASC, and pro- Caspase-1 expressions after 2 µM BaP exposure for two days in AhR-knockdown cells and control cells. Data are presented as the mean ± standard error. n = 4–7 independent experiments; *p<0.05, **p<0.01.

    Journal: Frontiers in immunology

    Article Title: Benzo[ a ]pyrene induces NLRP1 expression and promotes prolonged inflammasome signaling.

    doi: 10.3389/fimmu.2023.1154857

    Figure Lengend Snippet: FIGURE 3 AhR knockdown by siRNA abrogates the BaP-induced NLRP1 expression. AhR participation in the enhanced NLRP1 expression in BaP-treated A549 cells was determined using AhR KD cells and an AhR antagonist. (A) AhR mRNA and protein expression in response to specific siRNAs. (B) NLRP1 expression after 2 µM BaP exposure for two (mRNA) or four (protein) days in AhR-knockdown cells and control cells. (C) Pro-IL-1b, ASC, and pro- Caspase-1 expressions after 2 µM BaP exposure for two days in AhR-knockdown cells and control cells. Data are presented as the mean ± standard error. n = 4–7 independent experiments; *p<0.05, **p<0.01.

    Article Snippet: Monoclonal and polyclonal antibodies against NLRP1 (R&D Systems, Minneapolis, MN, USA), Frontiers in Immunology 03 NLRP3 (Abclonal Biotechnology, Wuhan, China), AhR (BioLegend, San Diego, CA), ASC (Abclonal Biotechnology), procaspase-1, p20, p53 (R&D Systems), phosphorylated p53 (Ser 37, Cell Signaling, Beverly, MA, USA), actin, and vinculin were primary antibodies.

    Techniques: Knockdown, Expressing, Control

    FIGURE 4 Effects of FICZ, a high-affinity ligand for AhR, on the NLRP1 expression in A549 cells. FICZ was dissolved in DMSO (final concentration, 0.05%) and added to A549 cells. NLRP-1, Pro-IL-1b, ASC, and pro-Caspase-1 expression at mRNA (A) and protein (B) levels. The cells were treated with 500 nM FICZ for 0–4 days. (C) CYP1A1 induction by BaP and FICZ treatment. Cells were treated with BaP (2 µM) and FICZ (500 nM) for four days. Data are presented as the mean ± standard error. n = 3–9 independent experiments. n.s., not significant.

    Journal: Frontiers in immunology

    Article Title: Benzo[ a ]pyrene induces NLRP1 expression and promotes prolonged inflammasome signaling.

    doi: 10.3389/fimmu.2023.1154857

    Figure Lengend Snippet: FIGURE 4 Effects of FICZ, a high-affinity ligand for AhR, on the NLRP1 expression in A549 cells. FICZ was dissolved in DMSO (final concentration, 0.05%) and added to A549 cells. NLRP-1, Pro-IL-1b, ASC, and pro-Caspase-1 expression at mRNA (A) and protein (B) levels. The cells were treated with 500 nM FICZ for 0–4 days. (C) CYP1A1 induction by BaP and FICZ treatment. Cells were treated with BaP (2 µM) and FICZ (500 nM) for four days. Data are presented as the mean ± standard error. n = 3–9 independent experiments. n.s., not significant.

    Article Snippet: Monoclonal and polyclonal antibodies against NLRP1 (R&D Systems, Minneapolis, MN, USA), Frontiers in Immunology 03 NLRP3 (Abclonal Biotechnology, Wuhan, China), AhR (BioLegend, San Diego, CA), ASC (Abclonal Biotechnology), procaspase-1, p20, p53 (R&D Systems), phosphorylated p53 (Ser 37, Cell Signaling, Beverly, MA, USA), actin, and vinculin were primary antibodies.

    Techniques: Expressing, Concentration Assay

    FIGURE 5 ROS generation is involved in the BaP-induced NLRP1 expression. The involvement of ROS was measured in BaP-treated A549 cells using NAC as an antioxidant. (A, B) NAC was added to the cells at a concentration of 5 mM 1 h prior to BaP application. Abrogated BaP-induced NLRP1 and p20 expression after NAC treatment were analyzed. Densitometry analysis of the western blot data of NLRP1 and p20 is shown in the bar graphs. Expression of NLRP1 mRNA (C) and protein (D) under H2O2 treatment (1 mM). (E) Analysis of components of the inflammasome protein complex in the H2O2-treated A549. The cells were then exposed to H2O2 at 1 mM for 9 h. Representative images (scale bar = 10 mm) of confocal microscopic observations of H2O2-treated cells. (F, G) AhR KD and control cells were exposed to BaP (F) at 2 µM and FICZ (G) at 500 nM for four days. The induced ROS were analyzed using flow cytometry, and the fold production of ROS was represented as the mean fluorescence intensity of DCF. Data are presented as the mean ± standard error. n = 3–4 independent experiments. *p<0.05, **p<0.01; NAC, N-acetyl-l-cysteine.; gMFI, geometric mean fluorescence intensity; DCF, dichlorofluorescein.

    Journal: Frontiers in immunology

    Article Title: Benzo[ a ]pyrene induces NLRP1 expression and promotes prolonged inflammasome signaling.

    doi: 10.3389/fimmu.2023.1154857

    Figure Lengend Snippet: FIGURE 5 ROS generation is involved in the BaP-induced NLRP1 expression. The involvement of ROS was measured in BaP-treated A549 cells using NAC as an antioxidant. (A, B) NAC was added to the cells at a concentration of 5 mM 1 h prior to BaP application. Abrogated BaP-induced NLRP1 and p20 expression after NAC treatment were analyzed. Densitometry analysis of the western blot data of NLRP1 and p20 is shown in the bar graphs. Expression of NLRP1 mRNA (C) and protein (D) under H2O2 treatment (1 mM). (E) Analysis of components of the inflammasome protein complex in the H2O2-treated A549. The cells were then exposed to H2O2 at 1 mM for 9 h. Representative images (scale bar = 10 mm) of confocal microscopic observations of H2O2-treated cells. (F, G) AhR KD and control cells were exposed to BaP (F) at 2 µM and FICZ (G) at 500 nM for four days. The induced ROS were analyzed using flow cytometry, and the fold production of ROS was represented as the mean fluorescence intensity of DCF. Data are presented as the mean ± standard error. n = 3–4 independent experiments. *p<0.05, **p<0.01; NAC, N-acetyl-l-cysteine.; gMFI, geometric mean fluorescence intensity; DCF, dichlorofluorescein.

    Article Snippet: Monoclonal and polyclonal antibodies against NLRP1 (R&D Systems, Minneapolis, MN, USA), Frontiers in Immunology 03 NLRP3 (Abclonal Biotechnology, Wuhan, China), AhR (BioLegend, San Diego, CA), ASC (Abclonal Biotechnology), procaspase-1, p20, p53 (R&D Systems), phosphorylated p53 (Ser 37, Cell Signaling, Beverly, MA, USA), actin, and vinculin were primary antibodies.

    Techniques: Expressing, Concentration Assay, Western Blot, Control, Cytometry

    FIGURE 6 Determination of the role of p53 in the BaP-induced NLRP1 expression. When p53 was knocked down using TP53-siRNA in A549 cells, BaP-induced NLRP1 expression was attenuated. (A) Phosphorylation of p53 in BaP-treated cells. Densitometry analysis of the western blot data of p53 phosphorylation is shown in the bar graph. (B) Expression levels of p53 in response to specific siRNAs. (C) Expression of NLRP1 mRNA and protein in p53 knockdown and control cells. BaP (2 µM) was added and incubated for four days. Data are presented as the mean ± standard error. n = 3–4 independent experiments. *p<0.05, **p<0.01, ***p<0.0001.

    Journal: Frontiers in immunology

    Article Title: Benzo[ a ]pyrene induces NLRP1 expression and promotes prolonged inflammasome signaling.

    doi: 10.3389/fimmu.2023.1154857

    Figure Lengend Snippet: FIGURE 6 Determination of the role of p53 in the BaP-induced NLRP1 expression. When p53 was knocked down using TP53-siRNA in A549 cells, BaP-induced NLRP1 expression was attenuated. (A) Phosphorylation of p53 in BaP-treated cells. Densitometry analysis of the western blot data of p53 phosphorylation is shown in the bar graph. (B) Expression levels of p53 in response to specific siRNAs. (C) Expression of NLRP1 mRNA and protein in p53 knockdown and control cells. BaP (2 µM) was added and incubated for four days. Data are presented as the mean ± standard error. n = 3–4 independent experiments. *p<0.05, **p<0.01, ***p<0.0001.

    Article Snippet: Monoclonal and polyclonal antibodies against NLRP1 (R&D Systems, Minneapolis, MN, USA), Frontiers in Immunology 03 NLRP3 (Abclonal Biotechnology, Wuhan, China), AhR (BioLegend, San Diego, CA), ASC (Abclonal Biotechnology), procaspase-1, p20, p53 (R&D Systems), phosphorylated p53 (Ser 37, Cell Signaling, Beverly, MA, USA), actin, and vinculin were primary antibodies.

    Techniques: Expressing, Phospho-proteomics, Western Blot, Knockdown, Control, Incubation

    FIGURE 8 Proposed scheme for regulating NLR proteins by BaP in the lung epithelial cells. We demonstrated that BaP enhances NLRP1 expression in lung epithelial A549 cells and leads to activation of the NLRP1 inflammasome cascade in the cells. At the same time, BaP also regulates the NLRP3 inflammasome through an independent pathway. There is a time gap between NLRP1 and the NLRP3 inflammasome caused by BaP.

    Journal: Frontiers in immunology

    Article Title: Benzo[ a ]pyrene induces NLRP1 expression and promotes prolonged inflammasome signaling.

    doi: 10.3389/fimmu.2023.1154857

    Figure Lengend Snippet: FIGURE 8 Proposed scheme for regulating NLR proteins by BaP in the lung epithelial cells. We demonstrated that BaP enhances NLRP1 expression in lung epithelial A549 cells and leads to activation of the NLRP1 inflammasome cascade in the cells. At the same time, BaP also regulates the NLRP3 inflammasome through an independent pathway. There is a time gap between NLRP1 and the NLRP3 inflammasome caused by BaP.

    Article Snippet: Monoclonal and polyclonal antibodies against NLRP1 (R&D Systems, Minneapolis, MN, USA), Frontiers in Immunology 03 NLRP3 (Abclonal Biotechnology, Wuhan, China), AhR (BioLegend, San Diego, CA), ASC (Abclonal Biotechnology), procaspase-1, p20, p53 (R&D Systems), phosphorylated p53 (Ser 37, Cell Signaling, Beverly, MA, USA), actin, and vinculin were primary antibodies.

    Techniques: Expressing, Activation Assay